Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234147_99lq GGTGGGAAGTCGAAGATGGGGAGTCTAAGAGG(+ACTCAC)GATCCTATAGGACCCGCCTGACCTGGGAATT NGG 10 8 17 8 24 0 100 5 10
    240604234146_mozk AGGACAATTCCCAGGTCAGGCGGGTCCTATAG(+ACTCAC)GATCCCTCTTAGACTCCCCATCTTCGACTTC NGG 10 8 17 8 24 0 100 5 10
    240604234145_cn1j GGTTTGTCCCTGAATCCCAGCCCTCCGGGTTG(+ACTCAC)GATCCGATCCTGTCAGGAGCAGTGGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604234143_ek1e GGAGGCAGACTCCACTGCTCCTGACAGGATCG(+ACTCAC)GATCCAACCCGGAGGGCTGGGATTCAGGGAC NGG 10 8 17 8 24 0 100 5 10
    240604234142_k15j TCCCCAGAGCCTGATACAGGTGACCTCGGAAG(+ACTCAC)GTACCAGCAGTAGGACCAGTGGACCGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240604234141_vtwa AAGCGGAGCACGGTCCACTGGTCCTACTGCTG(+ACTCAC)GTACCTTCCGAGGTCACCTGTATCAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240604234139_fgam ATGGAACCTTAACTGGGAGCTGCCGGATAAAG(+ACTCAC)GTACCCTTGCTCTCTGTGCTTATGGTTTGTC NGG 10 8 17 8 24 0 100 5 10
    240604234138_nouo TAGGTGACAAACCATAAGCACAGAGAGCAAGG(+ACTCAC)GTACCTTTATCCGGCAGCTCCCAGTTAAGGT NGG 10 8 17 8 24 0 100 5 10
    240604234136_siai GAGGCTCCTGAATTGTTTATTGTGTAGACATG(+ACTCAC)GATCCTGTGAAGGAAAAACTTAAACCCGAGG NGG 10 8 17 8 24 0 100 5 10
    240604234135_u3dw ATTACCCTCGGGTTTAAGTTTTTCCTTCACAG(+ACTCAC)GATCCATGTCTACACAATAAACAATTCAGGA NGG 10 8 17 8 24 0 100 5 10