Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234234_0jcl AGTTCCAGACACATCTTACCAAGCTGGGGCTG(+ACTCAC)GATCCAGGATGAAGTGGGGGAAGCAGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240604234232_0pzp CCTTGGCTTCCTGCTTCCCCCACTTCATCCTG(+ACTCAC)GATCCAGCCCCAGCTTGGTAAGATGTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604234231_0og7 TGAGAGGTTCTTTTCTCATCTGTTTTGAATCG(+ACTCAC)GTACCCTTAGTGGAAGGTCTGACGTGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604234229_u7yc TGCACTTTTCCACGTCAGACCTTCCACTAAGG(+ACTCAC)GTACCGATTCAAAACAGATGAGAAAAGAACC NGG 10 8 17 8 24 0 100 5 10
    240604234228_7dgw CCGATAGAGCCCCCGATGCTGAGTGCCAGCAG(+ACTCAC)GATCCTGTGTCCGACACCAGATCTGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240604234227_g1as ACAAAGATAAACAGATCTGGTGTCGGACACAG(+ACTCAC)GATCCTGCTGGCACTCAGCATCGGGGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240604234226_20bl AGGGTCTCATCATATAGACTTACATCTCTGAG(+ACTCAC)GTACCCTCTTGTGGTAATTCATTGTCATTAT NGG 10 8 17 8 24 0 100 5 10
    240604234224_yk9q AGTTGATAATGACAATGAATTACCACAAGAGG(+ACTCAC)GTACCTCAGAGATGTAAGTCTATATGATGAG NGG 10 8 17 8 24 0 100 5 10
    240604234222_rqxf TGAGTCACTTTCTATATGTGATAAAGCATGGG(+ACTCAC)GTACCTTCCCACTGGAGCGCAGCAAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240604234221_0453 AACACAGAGCCTTGCTGCGCTCCAGTGGGAAG(+ACTCAC)GTACCCCATGCTTTATCACATATAGAAAGTG NGG 10 8 17 8 24 0 100 5 10