Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604234249_z7g4 GGATGGACCATGTAGAGACTGCCATATCCAGG(+ACTCAC)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240604234247_m8jr GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ACTCAC)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604234245_8ad9 CACAGTTTAGTTTTTTGGGGGGAGTGGGTGGG(+ACTCAC)GTACCTTAGGTGTATTAACAGCAACAGATCT NGG 10 8 17 8 24 0 100 5 10
    240604234244_ae59 AAGTGAGATCTGTTGCTGTTAATACACCTAAG(+ACTCAC)GTACCCCACCCACTCCCCCCAAAAAACTAAA NGG 10 8 17 8 24 0 100 5 10
    240604234242_vtts AAGTTTGAGACTGTAAGAACTAATGTGTGCTG(+ACTCAC)GATCCACTGACTAACTCTCCAATTTTATATA NGG 10 8 17 8 24 0 100 5 10
    240604234241_fn3w TCTGATATATAAAATTGGAGAGTTAGTCAGTG(+ACTCAC)GATCCAGCACACATTAGTTCTTACAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240604234239_rf72 TTATTTTCTACTAGAAAATTCCATTTCCTCTG(+ACTCAC)GATCCAAGCTACATGAAATAAATCTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240604234238_d4x5 ACATACCTTTTAGATTTATTTCATGTAGCTTG(+ACTCAC)GATCCAGAGGAAATGGAATTTTCTAGTAGAA NGG 10 8 17 8 24 0 100 5 10
    240604234237_ogzg TCCCAAAGATTAGCCAGTAATTTGTAACTCAG(+ACTCAC)GATCCCACATCCATGGATTCAGCTTGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604234235_4v6j CCAGGTTTTTCAAGCTGAATCCATGGATGTGG(+ACTCAC)GATCCTGAGTTACAAATTACTGGCTAATCTT NGG 10 8 17 8 24 0 100 5 10