Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233949_dul2 CTAGGGTCCCATCGTTTGGTTAAATACCACGG(+ACTCAC)GTACCAGTTAACTCCTGGGCTCTAACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240604233948_gm7d GGACCTCTAGGTTAGAGCCCAGGAGTTAACTG(+ACTCAC)GTACCCGTGGTATTTAACCAAACGATGGGAC NGG 10 8 17 8 24 0 100 5 10
    240604233945_vhgf CCCTGTCCCAGCCAGCCATAGAGCCTTCCAGG(+ACTCAC)GATCCATCTTCTAAGCGCTCCGCCAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240604233944_ldws TCGACAGGGCCTGGCGGAGCGCTTAGAAGATG(+ACTCAC)GATCCCTGGAAGGCTCTATGGCTGGCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604233943_89xn TTCATTTATCCCATTATAAAACGGAGGTCAGG(+ACTCAC)GATCCCTCCGGGTATCTAACCACGGTCAACT NGG 10 8 17 8 24 0 100 5 10
    240604233941_rdso GGAAGAGTTGACCGTGGTTAGATACCCGGAGG(+ACTCAC)GATCCCTGACCTCCGTTTTATAATGGGATAA NGG 10 8 17 8 24 0 100 5 10
    240604233939_rvcg GCCAGATTTTCACGGGAAGGTCTTTAGTTCCG(+ACTCAC)GATCCTTGTAAGTATGAGTCCTTCATAGGGT NGG 10 8 17 8 24 0 100 5 10
    240604233937_v6u8 ATATAACCCTATGAAGGACTCATACTTACAAG(+ACTCAC)GATCCGGAACTAAAGACCTTCCCGTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240604233936_pgao GGATGCTCACCCAACATCTGCTTTCCTGGTTG(+ACTCAC)GATCCAGATTTGCACCATTTAAACATTGATG NGG 10 8 17 8 24 0 100 5 10
    240604233934_wodd ATGCTCATCAATGTTTAAATGGTGCAAATCTG(+ACTCAC)GATCCAACCAGGAAAGCAGATGTTGGGTGAG NGG 10 8 17 8 24 0 100 5 10