Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233809_ahlq GACAGCTCTGTCTGTCTGTCTGTCTCTCTTTG(+ACTCAC)GTACCAGGGATTACACCTACCTTGGGCCTTG NGG 10 8 17 8 24 0 100 5 10
    240604233806_jn0x ACACACAAGGCCCAAGGTAGGTGTAATCCCTG(+ACTCAC)GTACCAAAGAGAGACAGACAGACAGACAGAG NGG 10 8 17 8 24 0 100 5 10
    240604233804_mawi GCTCAGCTCAGCGAGGAGTCTGTAGCCTAGGG(+ACTCAC)GATCCAGTTCTGCTCTCAGGCATTAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240604233803_zc3y TTTTTTGTTTTTAATGCCTGAGAGCAGAACTG(+ACTCAC)GATCCCCTAGGCTACAGACTCCTCGCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240604233801_19g1 ACCCATTTAATCTCAGCACTTCAGAATCAATG(+ACTCAC)GATCCTTGAGTTTGAGGCCAGCCTGGGTACA NGG 10 8 17 8 24 0 100 5 10
    240604233800_wkvw TAGAATGTACCCAGGCTGGCCTCAAACTCAAG(+ACTCAC)GATCCATTGATTCTGAAGTGCTGAGATTAAA NGG 10 8 17 8 24 0 100 5 10
    240604233758_yf5z GACCTCAAGGACAACTCCTGAAAGCCAGCTGG(+ACTCAC)GATCCCAGGAACAGCAGTGAGACCCGGTCAT NGG 10 8 17 8 24 0 100 5 10
    240604233755_s2ed GTGGGATGACCGGGTCTCACTGCTGTTCCTGG(+ACTCAC)GATCCCAGCTGGCTTTCAGGAGTTGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240604233754_cutm TCAGAGGTGGGACAGAGAGCTCGGCCACTCAG(+ACTCAC)GATCCATCATGCAAGCAAGCAGGGTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604233752_gvi7 CGGCCGGAAGAACCCTGCTTGCTTGCATGATG(+ACTCAC)GATCCTGAGTGGCCGAGCTCTCTGTCCCACC NGG 10 8 17 8 24 0 100 5 10