Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232216_0g7l TGGAGTAGGGAAAAGGCCGACTCAGCCTAACG(+ACTCAC)GATCCCCCTGAGTCCCAGACAAAAGCCACTC NGG 10 8 17 8 24 0 100 5 10
    240604232214_z62w CAACTGAGTGGCTTTTGTCTGGGACTCAGGGG(+ACTCAC)GATCCGTTAGGCTGAGTCGGCCTTTTCCCTA NGG 10 8 17 8 24 0 100 5 10
    240604232213_kt14 GCTAATTTAATATTTGTATTTATCCATGCGTG(+ACTCAC)GATCCCTCTGCCACGCAACTGCTGGGTTATT NGG 10 8 17 8 24 0 100 5 10
    240604232211_5fhl TAATCAATAACCCAGCAGTTGCGTGGCAGAGG(+ACTCAC)GATCCACGCATGGATAAATACAAATATTAAA NGG 10 8 17 8 24 0 100 5 10
    240604232209_n81b TTCTGGCAGAAACCCCAAGATATTGCTCAAGG(+ACTCAC)GTACCCAATGCTACTTTACTTTCTATAAAGC NGG 10 8 17 8 24 0 100 5 10
    240604232207_yqh1 TACAGGCTTTATAGAAAGTAAAGTAGCATTGG(+ACTCAC)GTACCCTTGAGCAATATCTTGGGGTTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240604232206_ma6s CTGAAAATATGAAGAATTATTTTTATACAAAG(+ACTCAC)GATCCTTAAGCTTGGAGCACAATAAAGATGA NGG 10 8 17 8 24 0 100 5 10
    240604232204_jsha AGAGGTCATCTTTATTGTGCTCCAAGCTTAAG(+ACTCAC)GATCCTTTGTATAAAAATAATTCTTCATATT NGG 10 8 17 8 24 0 100 5 10
    240604232202_w4zd AAGCACTGAGTGCCATTAGCTAAATGCATAGG(+ACTCAC)GTACCACCCACAGGTGCCAGGGGCCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604232201_fvt8 ACTTTGGGAAAGGCCCCTGGCACCTGTGGGTG(+ACTCAC)GTACCCTATGCATTTAGCTAATGGCACTCAG NGG 10 8 17 8 24 0 100 5 10