Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232107_5t6k GAGAGAGGCAGCACTTGTCAGCGCCTGTGTAG(+ACTCAC)GTACCAGGGCATCAGAGCATGGCTTGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240604232105_yw5l TGGAAGCCAACAAGCCATGCTCTGATGCCCTG(+ACTCAC)GTACCTACACAGGCGCTGACAAGTGCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240604232103_mr79 GCAAGAACTGAACAGCAGCAACTGGTTTCTGG(+ACTCAC)GTACCAGAAAATGCCATTCACGATACGGCCC NGG 10 8 17 8 24 0 100 5 10
    240604232102_iyfz TGTTAGGGCCGTATCGTGAATGGCATTTTCTG(+ACTCAC)GTACCCAGAAACCAGTTGCTGCTGTTCAGTT NGG 10 8 17 8 24 0 100 5 10
    240604232101_wg24 ACCTGCCTACCACAAACACGGGTATATTCAAG(+ACTCAC)GTACCTGATACCTGGAGCAGGTTCTACACCT NGG 10 8 17 8 24 0 100 5 10
    240604232059_9co6 GGGGTAGGTGTAGAACCTGCTCCAGGTATCAG(+ACTCAC)GTACCTTGAATATACCCGTGTTTGTGGTAGG NGG 10 8 17 8 24 0 100 5 10
    240604232057_x96f ACCGCAGTTCCAGAAGCTTCGCCCTTCACAGG(+ACTCAC)GATCCACGCCCTCACTCGCTGTGTCACATGA NGG 10 8 17 8 24 0 100 5 10
    240604232056_i44t GCTCCTCATGTGACACAGCGAGTGAGGGCGTG(+ACTCAC)GATCCCTGTGAAGGGCGAAGCTTCTGGAACT NGG 10 8 17 8 24 0 100 5 10
    240604232053_zhpj CATGTTTGGAAAGCCCCACCCCTACAGCCCTG(+ACTCAC)GATCCTCTGTTCAGAATCAAAGCAGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240604232052_n8pf GCAGGGAAGGGCTGCTTTGATTCTGAACAGAG(+ACTCAC)GATCCAGGGCTGTAGGGGTGGGGCTTTCCAA NGG 10 8 17 8 24 0 100 5 10