Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232036_c97q TTATAGGGAAGATGATGTTAACACCTACTTTG(+ACTCAC)GTACCTTGACAAGTGTTACAGAGATCAACAA NGG 10 8 17 8 24 0 100 5 10
    240604232034_f87i CCCTTTTGTTGATCTCTGTAACACTTGTCAAG(+ACTCAC)GTACCAAAGTAGGTGTTAACATCATCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604232033_35mm GCTCCTTCCCTGTATCTCTCAGGCAGCAAGTG(+ACTCAC)GATCCCTCCGGACTAAAGGCCTAAGGTTTCA NGG 10 8 17 8 24 0 100 5 10
    240604232031_p4y9 GTCCCTGAAACCTTAGGCCTTTAGTCCGGAGG(+ACTCAC)GATCCACTTGCTGCCTGAGAGATACAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240604232030_3mtr TTTATAGCAAGAACAGTTCCCACTCCGAGCAG(+ACTCAC)GATCCTAAACTCCATCCCAAGTGAATACAGA NGG 10 8 17 8 24 0 100 5 10
    240604232029_pi2j AGAGATCTGTATTCACTTGGGATGGAGTTTAG(+ACTCAC)GATCCTGCTCGGAGTGGGAACTGTTCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240604232027_4qmk CCAGGCCGTGCTGGGGTGCTCTCTCTGCTGGG(+ACTCAC)GATCCTTATGGGGCCGGAGGCCACCTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604232026_qz6u ATGCTTTCCTAGGTGGCCTCCGGCCCCATAAG(+ACTCAC)GATCCCCAGCAGAGAGAGCACCCCAGCACGG NGG 10 8 17 8 24 0 100 5 10
    240604232024_opie TCTATGAGTATCTGTAAATGTTTTCTGAAGAG(+ACTCAC)GATCCACTGAGCAGCTGTAACCCATCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240604232023_4of0 CCACACTGGAGATGGGTTACAGCTGCTCAGTG(+ACTCAC)GATCCTCTTCAGAAAACATTTACAGATACTC NGG 10 8 17 8 24 0 100 5 10