Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232008_ak4v CAGTGAGCCAAAGCCCAGCGCCAGTTGTTGAG(+ACTCAC)GATCCAGAAATCGAGATGTCTGATACCACTA NGG 10 8 17 8 24 0 100 5 10
    240604232006_oai5 TGCCTTAGTGGTATCAGACATCTCGATTTCTG(+ACTCAC)GATCCTCAACAACTGGCGCTGGGCTTTGGCT NGG 10 8 17 8 24 0 100 5 10
    240604232004_kf8n CAAGAGTGGTGGTTGGGCAAGGAGGGCCCTTG(+ACTCAC)GTACCTAGACCATGGTTCCTTGCAACATAGA NGG 10 8 17 8 24 0 100 5 10
    240604232003_f7z3 CTCCCTCTATGTTGCAAGGAACCATGGTCTAG(+ACTCAC)GTACCAAGGGCCCTCCTTGCCCAACCACCAC NGG 10 8 17 8 24 0 100 5 10
    240604232001_ozln GAACTCTCCCTGGGTTCAGCCCTCTGGGGCGG(+ACTCAC)GTACCTCAGAGTGGGGGCTGGGTCACCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604232000_3pq4 TCTCTCTTGGGTGACCCAGCCCCCACTCTGAG(+ACTCAC)GTACCCGCCCCAGAGGGCTGAACCCAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240604231958_wzaa GGAGGGAGCTCCCCTGCACCGAGTAACTGGTG(+ACTCAC)GATCCCTGAAGTCACGGCAGCTCCGCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240604231956_7f2t CCTCCGCCTTGCGGAGCTGCCGTGACTTCAGG(+ACTCAC)GATCCACCAGTTACTCGGTGCAGGGGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240604231954_cfh5 CCCAGGTCTGGAACTTAGTAGACTGGGGTTGG(+ACTCAC)GTACCCAACCAATCAAACCCCTCCAAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240604231953_835n GTTCCAGGCTTTGGAGGGGTTTGATTGGTTGG(+ACTCAC)GTACCCAACCCCAGTCTACTAAGTTCCAGAC NGG 10 8 17 8 24 0 100 5 10