Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231920_6sra TGTGGGAGAAGAGCCCTGACCAAGCTGATCCG(+ACTCAC)GTACCTTGAAGGAAGTGCAATTGTTTCATGC NGG 10 8 17 8 24 0 100 5 10
    240604231918_ew95 AGTATGCATGAAACAATTGCACTTCCTTCAAG(+ACTCAC)GTACCGGATCAGCTTGGTCAGGGCTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240604231917_go2j AGCTAGAGTCTGGGTTGGAGAGGCCGAGTGAG(+ACTCAC)GATCCTTACAAGGCTACTGCATAGCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240604231916_8id7 TCCCGTGAGAAGCTATGCAGTAGCCTTGTAAG(+ACTCAC)GATCCTCACTCGGCCTCTCCAACCCAGACTC NGG 10 8 17 8 24 0 100 5 10
    240604231914_ere2 CACAGAAGTTATCTTGACCAAATGGCCTATTG(+ACTCAC)GTACCTACTCTTAATTTATTCTTTCAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240604231913_b24p GGGGAGTGCTTGAAAGAATAAATTAAGAGTAG(+ACTCAC)GTACCAATAGGCCATTTGGTCAAGATAACTT NGG 10 8 17 8 24 0 100 5 10
    240604231912_c6yx AAATAGCTCCCCACCTTCCCAAGGCTGTTATG(+ACTCAC)GTACCAGACACACAGCTCAGGACACCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604231910_wix1 TTCTCCCTGGGGTGTCCTGAGCTGTGTGTCTG(+ACTCAC)GTACCATAACAGCCTTGGGAAGGTGGGGAGC NGG 10 8 17 8 24 0 100 5 10
    240604231909_li6l CTCCATTACACCAAGGACACAGAAAAACACAG(+ACTCAC)GTACCGATTTGCCTTCCTCTCCTGCCAATCA NGG 10 8 17 8 24 0 100 5 10
    240604231908_7qi1 ACTTGTGATTGGCAGGAGAGGAAGGCAAATCG(+ACTCAC)GTACCTGTGTTTTTCTGTGTCCTTGGTGTAA NGG 10 8 17 8 24 0 100 5 10