Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231829_2fe9 GGAATTTGTGACAGCAGCCCTAGAAAACCAGG(+ACTCAC)GTACCTTCCACCTCCCCATCCCTCCATCTCA NGG 10 8 17 8 24 0 100 5 10
    240604231827_x6ig AAGGATGAGATGGAGGGATGGGGAGGTGGAAG(+ACTCAC)GTACCCTGGTTTTCTAGGGCTGCTGTCACAA NGG 10 8 17 8 24 0 100 5 10
    240604231825_nnj0 AGCGGCTCAGTGCCTGATGCCAGCAGCGGAAG(+ACTCAC)GTACCGTTTCCCACACGACAGACAGCCACAC NGG 10 8 17 8 24 0 100 5 10
    240604231824_bw1z CAACTGTGTGGCTGTCTGTCGTGTGGGAAACG(+ACTCAC)GTACCTTCCGCTGCTGGCATCAGGCACTGAG NGG 10 8 17 8 24 0 100 5 10
    240604231823_lv4d GTAACAATTAACCAGACTTCCCAAGACAAATG(+ACTCAC)GTACCAGCATCCTCATCCCAAGATGCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240604231822_c4z4 GTGGATTAAGGCATCTTGGGATGAGGATGCTG(+ACTCAC)GTACCATTTGTCTTGGGAAGTCTGGTTAATT NGG 10 8 17 8 24 0 100 5 10
    240604231820_p54e TTAATCTATTGCTTGGACTTAACTGGTTATTG(+ACTCAC)GATCCTTTGAGAAGAGAAATATTTACGATAT NGG 10 8 17 8 24 0 100 5 10
    240604231819_joks GTCATATATCGTAAATATTTCTCTTCTCAAAG(+ACTCAC)GATCCAATAACCAGTTAAGTCCAAGCAATAG NGG 10 8 17 8 24 0 100 5 10
    240604231818_mhqs TTAAGTACTTCATAACCTATTAAGGAAAACAG(+ACTCAC)GTACCTGGTGTCTCAGGAAGGCAATCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240604231816_kuda GTGTGATCTTGATTGCCTTCCTGAGACACCAG(+ACTCAC)GTACCTGTTTTCCTTAATAGGTTATGAAGTA NGG 10 8 17 8 24 0 100 5 10