Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231813_l84f CTGGAAGGAGTTAGCTCCCAGAGCTCCCCAGG(+ACTCAC)GTACCCTCCCGGGGTCGGCCTTACCTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240604231811_jx30 TGGAGATGGAAGGTAAGGCCGACCCCGGGAGG(+ACTCAC)GTACCCTGGGGAGCTCTGGGAGCTAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240604231809_pbyt TTGAGTTATGGATCGTTAGATCTTCATCCTGG(+ACTCAC)GATCCAGTTCCATTTTCTCTTCCGTCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240604231808_4bl0 TATCCTCAGGGACGGAAGAGAAAATGGAACTG(+ACTCAC)GATCCCAGGATGAAGATCTAACGATCCATAA NGG 10 8 17 8 24 0 100 5 10
    240604231807_5s35 GACAGTCCATCCATCCTTAACTGCAGAATCTG(+ACTCAC)GATCCCCTGCCCTCCAGTTTTGCTGCCGTTT NGG 10 8 17 8 24 0 100 5 10
    240604231805_a4v1 TTGATAAACGGCAGCAAAACTGGAGGGCAGGG(+ACTCAC)GATCCAGATTCTGCAGTTAAGGATGGATGGA NGG 10 8 17 8 24 0 100 5 10
    240604231803_mqoi ATATGTGTGTGTGTGTGTGTGTGTGTATATAG(+ACTCAC)GTACCTACAATCTTTGTGGAATAGATTCTAA NGG 10 8 17 8 24 0 100 5 10
    240604231802_hqzs TATGTTTAGAATCTATTCCACAAAGATTGTAG(+ACTCAC)GTACCTATATACACACACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240604231801_2yp8 GGAAGAGACCATCAAACTCATTGGGACTGGAG(+ACTCAC)GATCCTCAATGCTCTGCAAGAGCAGCCCATA NGG 10 8 17 8 24 0 100 5 10
    240604231759_z3ma GGTGTTATGGGCTGCTCTTGCAGAGCATTGAG(+ACTCAC)GATCCTCCAGTCCCAATGAGTTTGATGGTCT NGG 10 8 17 8 24 0 100 5 10