Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231906_ldjp CTTCTTTAATTCTTTCCCACAGGGAAGAATGG(+ACTCAC)GTACCTATCAAATTATGTGTGGTCAGGGTCA NGG 10 8 17 8 24 0 100 5 10
    240604231904_bc5r ACCAGTGACCCTGACCACACATAATTTGATAG(+ACTCAC)GTACCCATTCTTCCCTGTGGGAAAGAATTAA NGG 10 8 17 8 24 0 100 5 10
    240604231901_51wr AAAAACGTCTTTCCTCCACAACTCTAGCCCAG(+ACTCAC)GATCCGCCACACCTAAGTACATTACATCCTT NGG 10 8 17 8 24 0 100 5 10
    240604231859_l4hl ATGGAAAGGATGTAATGTACTTAGGTGTGGCG(+ACTCAC)GATCCTGGGCTAGAGTTGTGGAGGAAAGACG NGG 10 8 17 8 24 0 100 5 10
    240604231856_n36e GCCACTGAACCGACAGAGTTGCCCTAAGCCGG(+ACTCAC)GTACCTGACCTGAGTTCAGGGTAGCAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240604231855_h95w CTGTCTAGGCTGCTACCCTGAACTCAGGTCAG(+ACTCAC)GTACCCGGCTTAGGGCAACTCTGTCGGTTCA NGG 10 8 17 8 24 0 100 5 10
    240604231851_kv3s TTAGTGTTCACAGCAGTTAGAACTCACCCCTG(+ACTCAC)GATCCTGCAACCCATTTCCACTGATGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240604231850_l6p5 ATTTTAACCACATCAGTGGAAATGGGTTGCAG(+ACTCAC)GATCCAGGGGTGAGTTCTAACTGCTGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240604231848_hql9 GTCACCCCAAACCAAAGCGAAACACAGACCTG(+ACTCAC)GTACCGAAGACACTTCGTATTCCCAAAGCCG NGG 10 8 17 8 24 0 100 5 10
    240604231847_05fr GCACACGGCTTTGGGAATACGAAGTGTCTTCG(+ACTCAC)GTACCAGGTCTGTGTTTCGCTTTGGTTTGGG NGG 10 8 17 8 24 0 100 5 10