Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231933_fig2 GGCTCACAACCATCCATTACTCCAGTTCTCGG(+ACTCAC)GATCCACTGTCCCTAGCACTGACAGCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240604231932_vb9k GCCTGATGCTGCTGTCAGTGCTAGGGACAGTG(+ACTCAC)GATCCCGAGAACTGGAGTAATGGATGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240604231931_wbzh GAGAATACTATATTTGCATATCCATTAATCAG(+ACTCAC)GATCCAAAGCTGCTTAAATCGGGCGTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240604231930_rdjn GACTGGGAGGACGCCCGATTTAAGCAGCTTTG(+ACTCAC)GATCCTGATTAATGGATATGCAAATATAGTA NGG 10 8 17 8 24 0 100 5 10
    240604231928_v7g7 AACGTTCTGTACCAAACTGCCTGGAGGTTCTG(+ACTCAC)GATCCTCGCAGCTCTCGTAGTCATTGGGCGC NGG 10 8 17 8 24 0 100 5 10
    240604231927_ub27 CGGCTGCGCCCAATGACTACGAGAGCTGCGAG(+ACTCAC)GATCCAGAACCTCCAGGCAGTTTGGTACAGA NGG 10 8 17 8 24 0 100 5 10
    240604231926_n0ju ACAGACCCAGAGGGGGCGAGCCCTTGCTGCAG(+ACTCAC)GTACCAATTGGCTGAGCCATAGATCCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240604231925_3kzp TGGTGCCCCTGGATCTATGGCTCAGCCAATTG(+ACTCAC)GTACCTGCAGCAAGGGCTCGCCCCCTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604231923_iw5v AAGCTTTCTTAGAACTTGTTTTTATTCTTTGG(+ACTCAC)GTACCCAAAGGCCAAGTGATGAATCTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240604231921_biwr AATTCTTGCCAGATTCATCACTTGGCCTTTGG(+ACTCAC)GTACCCAAAGAATAAAAACAAGTTCTAAGAA NGG 10 8 17 8 24 0 100 5 10