Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232050_e24z AACACAAGGCTGCCATCCTGCAGCTCCAGAGG(+ACTCAC)GATCCCAGCCCTGGACCAAGGTCTGGCAACT NGG 10 8 17 8 24 0 100 5 10
    240604232048_bfoi TACTTAGTTGCCAGACCTTGGTCCAGGGCTGG(+ACTCAC)GATCCCTCTGGAGCTGCAGGATGGCAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240604232046_mp4s TTTTCAATTATAAGCAAGACAGGTGCAGACAG(+ACTCAC)GTACCATGAGACCCTCAGAGGCAGGAGTGCT NGG 10 8 17 8 24 0 100 5 10
    240604232045_w14m CCGCCAGCACTCCTGCCTCTGAGGGTCTCATG(+ACTCAC)GTACCTGTCTGCACCTGTCTTGCTTATAATT NGG 10 8 17 8 24 0 100 5 10
    240604232044_rg5t GGTTCCCTTCCAGTATGCAGTTAATCATGATG(+ACTCAC)GATCCTGTATATACGAGCTCCTCCGTCCCCT NGG 10 8 17 8 24 0 100 5 10
    240604232043_7f1f GCTGAAGGGGACGGAGGAGCTCGTATATACAG(+ACTCAC)GATCCATCATGATTAACTGCATACTGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604232042_qmuk GGGAGGCTGTAAAATGGCCCATGTATATGTAG(+ACTCAC)GATCCTGGATGGTCTACATATATGTTAATAC NGG 10 8 17 8 24 0 100 5 10
    240604232040_82b3 TTCATGTATTAACATATATGTAGACCATCCAG(+ACTCAC)GATCCTACATATACATGGGCCATTTTACAGC NGG 10 8 17 8 24 0 100 5 10
    240604232038_fesr GGACACCCTGTAACCTTACTGCTTCAGGGCTG(+ACTCAC)GATCCAGACTAAGAGGTTGTGAGGTTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240604232037_qwmk CTGCATCCCCAACCTCACAACCTCTTAGTCTG(+ACTCAC)GATCCAGCCCTGAAGCAGTAAGGTTACAGGG NGG 10 8 17 8 24 0 100 5 10