Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232124_gveg GGAGTACCCGTTGAAGACTGGGCCGATCTAGG(+ACTCAC)GTACCTAGCTTCAAGGTCTGTTGCAGTAAAT NGG 10 8 17 8 24 0 100 5 10
    240604232123_97ln TGGAGATTTACTGCAACAGACCTTGAAGCTAG(+ACTCAC)GTACCCTAGATCGGCCCAGTCTTCAACGGGT NGG 10 8 17 8 24 0 100 5 10
    240604232121_0jb0 ATGTCCCAGACACACAGGACCAGCTAAGGCTG(+ACTCAC)GATCCCTCTTCAGAGAGATTGGGGAGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240604232119_vk57 AAGCCTCTCACTCCCCAATCTCTCTGAAGAGG(+ACTCAC)GATCCAGCCTTAGCTGGTCCTGTGTGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240604232118_2bsu TGGGCCTGGCCTGGCCAGTGCTTGGTGGAAAG(+ACTCAC)GATCCACAGCTCCCTGGGGCCCCAAGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240604232117_krfg TTCACCACAACTTGGGGCCCCAGGGAGCTGTG(+ACTCAC)GATCCTTTCCACCAAGCACTGGCCAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604232114_l3tj GGCACAGCTGACCAGTGCAGGCTGACGCCCTG(+ACTCAC)GATCCTGAGCTCTCGTGTGGAACTGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240604232113_96l8 AGCAGCAGCTCCAGTTCCACACGAGAGCTCAG(+ACTCAC)GATCCAGGGCGTCAGCCTGCACTGGTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240604232111_di28 CAGGTTGTCCCTGCTGAGGATCTGGTCACTGG(+ACTCAC)GATCCAGGGTGGCTGGGCGAGAAGAGGAACC NGG 10 8 17 8 24 0 100 5 10
    240604232109_61ba CCAGGGGTTCCTCTTCTCGCCCAGCCACCCTG(+ACTCAC)GATCCCAGTGACCAGATCCTCAGCAGGGACA NGG 10 8 17 8 24 0 100 5 10