Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225443_aace TGCCACCACCAGAGTCCATGCACCTCAACGTG(+ACTCAC)GATCCACTTGGGTCCTGGCATCTTGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240604225442_27zk ATAAATCACCTCAAGATGCCAGGACCCAAGTG(+ACTCAC)GATCCACGTTGAGGTGCATGGACTCTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240604225439_xulf TTCTGCTTCTTACCCTCCCTTCCAAGCCCTGG(+ACTCAC)GATCCTAAAAGTCCCGCCTTTGTCTGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240604225437_r8sr CTAGGCAGGACAGACAAAGGCGGGACTTTTAG(+ACTCAC)GATCCCAGGGCTTGGAAGGGAGGGTAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604225436_m7vz GATTCTCTGCTCTTTGTATGACCGGGTGAAAG(+ACTCAC)GTACCTGTCGTCTTTACAAGTTAGTTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240604225435_3g2w ATACAGTCCAAACTAACTTGTAAAGACGACAG(+ACTCAC)GTACCTTTCACCCGGTCATACAAAGAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240604225434_hohp TATAAGTTTAGTATACAATGGATCAAATTAAG(+ACTCAC)GATCCTGCTCTCTTTCCTTTGGAGGTAGTTC NGG 10 8 17 8 24 0 100 5 10
    240604225432_hkmx ACGTCGAACTACCTCCAAAGGAAAGAGAGCAG(+ACTCAC)GATCCTTAATTTGATCCATTGTATACTAAAC NGG 10 8 17 8 24 0 100 5 10
    240604225430_n6tl CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+ACTCAC)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604225428_4iaa GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+ACTCAC)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10