Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225558_wzca ACCACATAGTGGCTCATAACCATCTGTAATGG(+ACTCAC)GATCCAATGCCATCTTCTGGTATCTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604225557_wt9i GCTGTCTTCAGAGATACCAGAAGATGGCATTG(+ACTCAC)GATCCCATTACAGATGGTTATGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240604225556_rr7u AGTCACCGCCTGGCAAGGGCCATGGTAGTTAG(+ACTCAC)GTACCGAAATGCTCCTGTACAGATGCAAACC NGG 10 8 17 8 24 0 100 5 10
    240604225555_3i6e GTACAGGTTTGCATCTGTACAGGAGCATTTCG(+ACTCAC)GTACCTAACTACCATGGCCCTTGCCAGGCGG NGG 10 8 17 8 24 0 100 5 10
    240604225552_tiln CTAGCTGGGAGTGCCGGCAGAGGCAGGGCTGG(+ACTCAC)GTACCACAGGGCCAGCTTGAGTGTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240604225549_i562 CAAGTTCCTCCCACACTCAAGCTGGCCCTGTG(+ACTCAC)GTACCCAGCCCTGCCTCTGCCGGCACTCCCA NGG 10 8 17 8 24 0 100 5 10
    240604225547_g9hp TCCATGCCTCCCCACCCTTGTTCACGCCCATG(+ACTCAC)GATCCCCTGGCCTTGATGTCTGCCCCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240604225544_w9w1 CTGAGAAAGAGGGGCAGACATCAAGGCCAGGG(+ACTCAC)GATCCATGGGCGTGAACAAGGGTGGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240604225542_a56b CTCCCCAGAGGACGCAGTGAGTCCCCAGCTGG(+ACTCAC)GATCCCACCACACTGGACAGGAGCCAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240604225539_rl24 AGAGCACTCCTGGCTCCTGTCCAGTGTGGTGG(+ACTCAC)GATCCCAGCTGGGGACTCACTGCGTCCTCTG NGG 10 8 17 8 24 0 100 5 10