Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225538_yf61 TGACTCCTTCAGTGTCTATAGAAGAAAAGGTG(+ACTCAC)GATCCTGGAGCCTACAGAAAACTCAACCCTT NGG 10 8 17 8 24 0 100 5 10
    240604225536_qb3t CCTAGAAGGGTTGAGTTTTCTGTAGGCTCCAG(+ACTCAC)GATCCACCTTTTCTTCTATAGACACTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604225534_0moj TGACTCCTTCAGTGTCTATAGAAGAAAAGGTG(+ACTCAC)GATCCTGGAGCCTACAGAAAACTCAACCCTT NGG 10 8 17 8 24 0 100 5 10
    240604225532_fjp3 CCTAGAAGGGTTGAGTTTTCTGTAGGCTCCAG(+ACTCAC)GATCCACCTTTTCTTCTATAGACACTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604225529_tf4d GGGAGCCAGTGGTTTCTGAAGACTGTCCAGGG(+ACTCAC)GATCCCTAGTTCAGAGTGGCCCCATCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604225527_ajcg CATGTTGCCTGATGGGGCCACTCTGAACTAGG(+ACTCAC)GATCCCCTGGACAGTCTTCAGAAACCACTGG NGG 10 8 17 8 24 0 100 5 10
    240604225525_kysx AGGGAGGTGTGCGATGTTAGCCTTCTGCTTGG(+ACTCAC)GATCCTTTGACCGGGAGGCCTGTAGTGTCCT NGG 10 8 17 8 24 0 100 5 10
    240604225524_38fu ACTTCAGGACACTACAGGCCTCCCGGTCAAAG(+ACTCAC)GATCCCAAGCAGAAGGCTAACATCGCACACC NGG 10 8 17 8 24 0 100 5 10
    240604225521_c0ik GGCCCTGCTAGATGCTGACATCAGCCCCAGGG(+ACTCAC)GTACCTTTCTGCTCTGCAGGACTGATCTCAT NGG 10 8 17 8 24 0 100 5 10
    240604225520_lbgh GGAGGATGAGATCAGTCCTGCAGAGCAGAAAG(+ACTCAC)GTACCCCTGGGGCTGATGTCAGCATCTAGCA NGG 10 8 17 8 24 0 100 5 10