Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225616_du41 CAGACTGGGAGGGGACGGGTAAGAGAGGTGGG(+ACTCAC)GTACCAGACTCCAACAGGCTCCAAAGAAATA NGG 10 8 17 8 24 0 100 5 10
    240604225614_8p3x TTTTCTATTTCTTTGGAGCCTGTTGGAGTCTG(+ACTCAC)GTACCCCACCTCTCTTACCCGTCCCCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240604225612_p42s GTAGGGCCAGCGGGCGCCGGAAGTGATTCCAG(+ACTCAC)GTACCCTTTCCCCCACCTTGGAGTCTGACAA NGG 10 8 17 8 24 0 100 5 10
    240604225610_iaol GAGACTTGTCAGACTCCAAGGTGGGGGAAAGG(+ACTCAC)GTACCTGGAATCACTTCCGGCGCCCGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240604225608_5w7o AGCTTGCCCTCCTCCCTGCCAGGGCGGGGAAG(+ACTCAC)GATCCCTTCTGTTCCTTGTGGACTCCGAGCT NGG 10 8 17 8 24 0 100 5 10
    240604225607_xwix GCAGTAGCTCGGAGTCCACAAGGAACAGAAGG(+ACTCAC)GATCCTTCCCCGCCCTGGCAGGGAGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604225605_fnao AGCTTGCCCTCCTCCCTGCCAGGGCGGGGAAG(+ACTCAC)GATCCCTTCTGTTCCTTGTGGACTCCGAGCT NGG 10 8 17 8 24 0 100 5 10
    240604225603_i2c4 GCAGTAGCTCGGAGTCCACAAGGAACAGAAGG(+ACTCAC)GATCCTTCCCCGCCCTGGCAGGGAGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604225602_9p88 GCTGAAGTTACAGCTCTACCTGCCTGATTTGG(+ACTCAC)GATCCAAACTCTGCAAGCAGGGAGTGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240604225600_71wq CAATTAAGAGCACTCCCTGCTTGCAGAGTTTG(+ACTCAC)GATCCCAAATCAGGCAGGTAGAGCTGTAACT NGG 10 8 17 8 24 0 100 5 10