Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225722_82z4 GGTCCCTAGTTTGCAAATTCAGTGTTTGGGTG(+ACTCAC)GATCCGTTCCTAGGGTACCTAAGATGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240604225721_w4t5 CTCCCTCAAACATCTTAGGTACCCTAGGAACG(+ACTCAC)GATCCACCCAAACACTGAATTTGCAAACTAG NGG 10 8 17 8 24 0 100 5 10
    240604225719_846w CTGGAGATTGACCTGTGTAAAGTAACGTTTAG(+ACTCAC)GATCCTGACGTGACTGTCATCTCCAAGACTT NGG 10 8 17 8 24 0 100 5 10
    240604225717_4ejt GGAGGAAGTCTTGGAGATGACAGTCACGTCAG(+ACTCAC)GATCCTAAACGTTACTTTACACAGGTCAATC NGG 10 8 17 8 24 0 100 5 10
    240604225716_be4q GTGACTCTGGGGTTCAAGGCAGAGACTATGTG(+ACTCAC)GATCCCAGGGTCATACCGCCAAGCCAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240604225713_h4ex TCAGCTAGACTGGCTTGGCGGTATGACCCTGG(+ACTCAC)GATCCACATAGTCTCTGCCTTGAACCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240604225711_7932 TCAGCACCCAAGATTGGGGGATAACTAGACTG(+ACTCAC)GTACCAGTTCATATATGCCTTATAAAATCTG NGG 10 8 17 8 24 0 100 5 10
    240604225710_zgoc TCAAACAGATTTTATAAGGCATATATGAACTG(+ACTCAC)GTACCAGTCTAGTTATCCCCCAATCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240604225708_4t0n TCTGTACCCACTGTCTGTCTGCTCCCCCGATG(+ACTCAC)GTACCTAAGGCAGCCTGCTGAATATACCCAC NGG 10 8 17 8 24 0 100 5 10
    240604225705_058r AGGTTGTGGGTATATTCAGCAGGCTGCCTTAG(+ACTCAC)GTACCATCGGGGGAGCAGACAGACAGTGGGT NGG 10 8 17 8 24 0 100 5 10