Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225835_9npz AGGGCTTGTTGAGTAGGCATCTGGAATGACCG(+ACTCAC)GATCCGGGCATCAGGCCGCTGATGGGCATAG NGG 10 8 17 8 24 0 100 5 10
    240604225833_6w99 CCACTCTATGCCCATCAGCGGCCTGATGCCCG(+ACTCAC)GATCCGGTCATTCCAGATGCCTACTCAACAA NGG 10 8 17 8 24 0 100 5 10
    240604225832_5b5a GCTACCCAGAGGCCTTTCGTCTCCCTGTTCTG(+ACTCAC)GTACCCAGCTTCCCAATCAGCAGCCAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240604225830_wqob TGTTGAGCTCTGGCTGCTGATTGGGAAGCTGG(+ACTCAC)GTACCAGAACAGGGAGACGAAAGGCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240604225828_shvg CAGTGTGGATCTGCGGGTTGTCACTAGTCCAG(+ACTCAC)GTACCGAGGACAGACGTTATAGAGTGACACT NGG 10 8 17 8 24 0 100 5 10
    240604225826_b98r TGGACAGTGTCACTCTATAACGTCTGTCCTCG(+ACTCAC)GTACCTGGACTAGTGACAACCCGCAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240604225825_hq8l CCACCAGCTAGCCTGGCTTGACTGTGGGATTG(+ACTCAC)GATCCTTGCAGAGAGCTTTCGCTTTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240604225824_jjme TGTGAGTGCTCAAAGCGAAAGCTCTCTGCAAG(+ACTCAC)GATCCAATCCCACAGTCAAGCCAGGCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240604225821_qe11 AGCAAAACAAAGTGTTTGTGTCCCTGCCACGG(+ACTCAC)GATCCTATCATTTCATCTGTCTTCTCATTGG NGG 10 8 17 8 24 0 100 5 10
    240604225820_iyqn ATGAACCAATGAGAAGACAGATGAAATGATAG(+ACTCAC)GATCCCGTGGCAGGGACACAAACACTTTGTT NGG 10 8 17 8 24 0 100 5 10