Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225819_uvwo CTGGTTCTTGCAGAGGCCAGAGGGCAGCACTG(+ACTCAC)GATCCCCTGGAACTACCATTACAGCCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240604225816_rcve GCTCACAACTGGCTGTAATGGTAGTTCCAGGG(+ACTCAC)GATCCAGTGCTGCCCTCTGGCCTCTGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240604225815_kiin CTGGCTCCCATCCGAAAGCTTAGCTAGAGAAG(+ACTCAC)GATCCACATTGGAGGGAACAGGTTAGACACC NGG 10 8 17 8 24 0 100 5 10
    240604225814_mn1o ATTCAGGTGTCTAACCTGTTCCCTCCAATGTG(+ACTCAC)GATCCTTCTCTAGCTAAGCTTTCGGATGGGA NGG 10 8 17 8 24 0 100 5 10
    240604225812_rlzd GCTTGCTGTGTAAGCATGAGGACCCAACTTTG(+ACTCAC)GATCCCCAGAACTCAAGTTAACTGCCAGGTA NGG 10 8 17 8 24 0 100 5 10
    240604225810_i5wz ACATCTACCTGGCAGTTAACTTGAGTTCTGGG(+ACTCAC)GATCCAAAGTTGGGTCCTCATGCTTACACAG NGG 10 8 17 8 24 0 100 5 10
    240604225809_2zk6 GTTTAATTCAGTGCTACGGAGACAGGCAAGTG(+ACTCAC)GATCCCTGGGGTTCACTGGCCAGTTAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240604225805_3v21 TAGGCTCAGCTAACTGGCCAGTGAACCCCAGG(+ACTCAC)GATCCACTTGCCTGTCTCCGTAGCACTGAAT NGG 10 8 17 8 24 0 100 5 10
    240604225804_5nui CCACAACTGCGTAACACATAGTACTAAGAGAG(+ACTCAC)GTACCAGGGACTGCACTTGTATGACACTCAG NGG 10 8 17 8 24 0 100 5 10
    240604225801_50fl CAGTCCTGAGTGTCATACAAGTGCAGTCCCTG(+ACTCAC)GTACCTCTCTTAGTACTATGTGTTACGCAGT NGG 10 8 17 8 24 0 100 5 10