Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225912_cb71 CTATTAGGAGAATAGATGTTAATGTGTTTTGG(+ACTCAC)GTACCCATCTTTGCAACATGTCTGATCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604225910_pnnp TGGCACTTGGATCAGACATGTTGCAAAGATGG(+ACTCAC)GTACCCAAAACACATTAACATCTATTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240604225908_57ov CTCAGATCCAGTCCACGGGTCCAAGGATGCTG(+ACTCAC)GATCCAAGGACTTCTCTTCCACAGTGGCCGG NGG 10 8 17 8 24 0 100 5 10
    240604225906_0b2i GTGGGCCGGCCACTGTGGAAGAGAAGTCCTTG(+ACTCAC)GATCCAGCATCCTTGGACCCGTGGACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240604225904_zsm3 CTGTTCTTGCCCCCGTCACATTCTGGAAAGGG(+ACTCAC)GTACCCAGTGATCTCTGTGACGCCTCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240604225902_43kc GAGGAGTCTGGAGGCGTCACAGAGATCACTGG(+ACTCAC)GTACCCCTTTCCAGAATGTGACGGGGGCAAG NGG 10 8 17 8 24 0 100 5 10
    240604225900_tih3 TTTAAACCTTTCAAAACAAAATTCCTATCCAG(+ACTCAC)GATCCCATCCAGAGTCATCACCTGCCACTCA NGG 10 8 17 8 24 0 100 5 10
    240604225858_yoi3 CTTGTTGAGTGGCAGGTGATGACTCTGGATGG(+ACTCAC)GATCCTGGATAGGAATTTTGTTTTGAAAGGT NGG 10 8 17 8 24 0 100 5 10
    240604225857_em05 GGGAGGCCATGCCCGCCACCCCTCTTGTTCTG(+ACTCAC)GATCCCTGCTCCCCTCTGGGTGTGTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240604225855_8ynf CACACACACCCACACACCCAGAGGGGAGCAGG(+ACTCAC)GATCCAGAACAAGAGGGGTGGCGGGCATGGC NGG 10 8 17 8 24 0 100 5 10