Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230003_gyvx CTGAGAACTCGCTAGGCAAAGAGAAGCTCTTG(+ACTCAC)GATCCTGGCACTCACCTTAAGTCTTTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240604230001_k5ey ACAAGTCTACAAAGACTTAAGGTGAGTGCCAG(+ACTCAC)GATCCAAGAGCTTCTCTTTGCCTAGCGAGTT NGG 10 8 17 8 24 0 100 5 10
    240604225959_eo30 TCTTAGGCCACTGTGGCAAGAACTAGGAAGGG(+ACTCAC)GATCCACATCAGAATGCCCTAGCTGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240604225958_wfbd GAGATCCCTGCCAGCTAGGGCATTCTGATGTG(+ACTCAC)GATCCCCTTCCTAGTTCTTGCCACAGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240604225957_cpi3 AGTTTAATTGCAGAACTGGACCATATCTTTAG(+ACTCAC)GATCCTGTAGGGGAGACAGACATGAATCAAA NGG 10 8 17 8 24 0 100 5 10
    240604225955_aniv GGTGGTTTGATTCATGTCTGTCTCCCCTACAG(+ACTCAC)GATCCTAAAGATATGGTCCAGTTCTGCAATT NGG 10 8 17 8 24 0 100 5 10
    240604225954_5x24 GAGGTGGGAAATGGCCAGAAATCAAGCTGAAG(+ACTCAC)GATCCACCTCCATGTAACCACAGCGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240604225952_axg9 GTGTTTCCTGTCGCTGTGGTTACATGGAGGTG(+ACTCAC)GATCCTTCAGCTTGATTTCTGGCCATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604225948_fb9z ATCATCACCTTACAGATGTGCCACGCGAAGCG(+ACTCAC)GATCCATTCACCAATGCCTGTGTTCTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240604225946_vhar TTCTGGTCACAGAACACAGGCATTGGTGAATG(+ACTCAC)GATCCGCTTCGCGTGGCACATCTGTAAGGTG NGG 10 8 17 8 24 0 100 5 10