Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230039_cngl CCACAGCACAGAGATACCCACCAGTGTTCCAG(+ACTCAC)GTACCCAGCAAGCTAACTAGCATAGACCTGG NGG 10 8 17 8 24 0 100 5 10
    240604230037_gx0b TTATCCCAGGTCTATGCTAGTTAGCTTGCTGG(+ACTCAC)GTACCTGGAACACTGGTGGGTATCTCTGTGC NGG 10 8 17 8 24 0 100 5 10
    240604230036_klu5 TGATATATTTCCCTACCTGCATAGCATGAATG(+ACTCAC)GTACCCAGCTGCTATCTTGTGTTTCAACAAT NGG 10 8 17 8 24 0 100 5 10
    240604230034_vgom AAGCCATTGTTGAAACACAAGATAGCAGCTGG(+ACTCAC)GTACCATTCATGCTATGCAGGTAGGGAAATA NGG 10 8 17 8 24 0 100 5 10
    240604230032_p6vs GACCCACATGCATATATTAGCCAGAGCTTGGG(+ACTCAC)GTACCCTGTGTAAGAGTAGTAGGATGGATTG NGG 10 8 17 8 24 0 100 5 10
    240604230030_r3rw TCTTACAATCCATCCTACTACTCTTACACAGG(+ACTCAC)GTACCCCAAGCTCTGGCTAATATATGCATGT NGG 10 8 17 8 24 0 100 5 10
    240604230028_rona GAATACCACATGCGTTTTTGTCTAATGCTTAG(+ACTCAC)GTACCAAAATGTTAGCATCAGTATTCACTAT NGG 10 8 17 8 24 0 100 5 10
    240604230027_ypfb GCTCTATAGTGAATACTGATGCTAACATTTTG(+ACTCAC)GTACCTAAGCATTAGACAAAAACGCATGTGG NGG 10 8 17 8 24 0 100 5 10
    240604230026_lyyf TGGTATCTGGAAGTCTGTGCACAAGATCTGAG(+ACTCAC)GTACCATTGGGTGGGCTGAGATCTTTAAGTG NGG 10 8 17 8 24 0 100 5 10
    240604230024_y1o7 TGGGACACTTAAAGATCTCAGCCCACCCAATG(+ACTCAC)GTACCTCAGATCTTGTGCACAGACTTCCAGA NGG 10 8 17 8 24 0 100 5 10