Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230149_5p64 CTGTCACCCAGCACTGGCAAGGAAGACAGGAG(+ACTCAC)GATCCATGAGGCTGACTGGCTAGCAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240604230148_rqg4 AGCACTAGGCTTGCTAGCCAGTCAGCCTCATG(+ACTCAC)GATCCTCCTGTCTTCCTTGCCAGTGCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240604230146_31np AAAAGGAGGGTTGTTGGGGTGAGGAGAAAAGG(+ACTCAC)GTACCCCTCGAAGAAGCTCCTCAACCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240604230144_nndu ACCTTCCAGGGGTTGAGGAGCTTCTTCGAGGG(+ACTCAC)GTACCCTTTTCTCCTCACCCCAACAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240604230143_f4tc CACAGCAGCACAAACGGACTGTGGAGACACAG(+ACTCAC)GTACCAGTTCATGAATATTCTGGGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240604230142_m47e GAGAGATAGAAACCCAGAATATTCATGAACTG(+ACTCAC)GTACCTGTGTCTCCACAGTCCGTTTGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240604230140_p8bq CCATACCCCCAAGACTCCCCTTGGCCACAGTG(+ACTCAC)GTACCCTCCAGCTGCCCTTGGGCCCCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604230138_buzc GCCAGCTGCTGGGGCCCAAGGGCAGCTGGAGG(+ACTCAC)GTACCACTGTGGCCAAGGGGAGTCTTGGGGG NGG 10 8 17 8 24 0 100 5 10
    240604230136_74m4 TAACAGTGAACACAGCAAGGTCTGTGTTATGG(+ACTCAC)GATCCTTTTCCCAAAACAAACAAACAAACAA NGG 10 8 17 8 24 0 100 5 10
    240604230135_hakm TTTGTTTGTTTGTTTGTTTGTTTTGGGAAAAG(+ACTCAC)GATCCCATAACACAGACCTTGCTGTGTTCAC NGG 10 8 17 8 24 0 100 5 10