Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230115_emdo TTCTGGATATATGCCCAGGAGAGGTATTGCGG(+ACTCAC)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240604230113_p5sl TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ACTCAC)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240604230110_se1l AGGAAAGTGATCCAGAGACTGCCATATCTGGG(+ACTCAC)GATCCATCCTGTATACAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240604230109_zqxe AGTGTCTGGGTTTGGTGACTGTATACAGGATG(+ACTCAC)GATCCCCAGATATGGCAGTCTCTGGATCACT NGG 10 8 17 8 24 0 100 5 10
    240604230106_ku25 TTCATGTATGTGTATGTATGTGTATGTCCCTG(+ACTCAC)GTACCCCTAGAGGCTGAAAAAAGGTGTCACA NGG 10 8 17 8 24 0 100 5 10
    240604230104_7mpi GAGAATGTGACACCTTTTTTCAGCCTCTAGGG(+ACTCAC)GTACCAGGGACATACACATACATACACATAC NGG 10 8 17 8 24 0 100 5 10
    240604230102_eqya GGCAGTGGAATACTGCTACAGGATTGAGATGG(+ACTCAC)GATCCTGGAACAATTTGGTGGAACATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240604230100_ojd7 TAGTGTTCTTATGTTCCACCAAATTGTTCCAG(+ACTCAC)GATCCCATCTCAATCCTGTAGCAGTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240604230059_n3ig ATGCCAAATGTTTTTTTGATACATATTTGTTG(+ACTCAC)GTACCATGCCTAAAATATACAATGAAGCGAC NGG 10 8 17 8 24 0 100 5 10
    240604230057_97of GGAATGTCGCTTCATTGTATATTTTAGGCATG(+ACTCAC)GTACCAACAAATATGTATCAAAAAAACATTT NGG 10 8 17 8 24 0 100 5 10