Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230134_ykf9 CTGGGGAGACTGTAGGAATGATATTTCTCAAG(+ACTCAC)GTACCTCTGAAGCTGTAAGAACACTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240604230132_wfje TCCCAGAGCCCAGTGTTCTTACAGCTTCAGAG(+ACTCAC)GTACCTTGAGAAATATCATTCCTACAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240604230131_cuof ATCAATGAGAGTCATTGATCTCGCACCATGTG(+ACTCAC)GATCCTGGGGATTGAATTTGGATTTATTAGA NGG 10 8 17 8 24 0 100 5 10
    240604230127_f9f4 CCAAGTCTAATAAATCCAAATTCAATCCCCAG(+ACTCAC)GATCCACATGGTGCGAGATCAATGACTCTCA NGG 10 8 17 8 24 0 100 5 10
    240604230126_92yb TCTCTCCAGTCTCCATTATCTGTTCTTTTATG(+ACTCAC)GATCCCAAAAGATTAGTCATATACAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240604230124_izpf GGTAAAGCCTTTGTATATGACTAATCTTTTGG(+ACTCAC)GATCCATAAAAGAACAGATAATGGAGACTGG NGG 10 8 17 8 24 0 100 5 10
    240604230122_3c02 ATGAAATGGACAAATTTCTAGACAGATACCAG(+ACTCAC)GTACCAAAGTTAAATCAGGATCAAGTTAACG NGG 10 8 17 8 24 0 100 5 10
    240604230120_ybsv TAGATCGTTAACTTGATCCTGATTTAACTTTG(+ACTCAC)GTACCTGGTATCTGTCTAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240604230119_kdxt GGTGTCAGCATTTGGAGGCTGATTATGGGATG(+ACTCAC)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604230116_sga4 GGATGGACCATGTAGAGACTGCCATATCCAGG(+ACTCAC)GATCCATCCCATAATCAGCCTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10