Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225649_d9zn AAAGCCTTGTGACATAGCTGTCCAAACATTTG(+ACTCAC)GATCCTTTAGCCACAGGATGACCCACTTGTC NGG 10 8 17 8 24 0 100 5 10
    240604225648_gnj2 TCTTAGACAAGTGGGTCATCCTGTGGCTAAAG(+ACTCAC)GATCCAAATGTTTGGACAGCTATGTCACAAG NGG 10 8 17 8 24 0 100 5 10
    240604225646_x2sp TATTTCTCTCATTTGAAGATTTTCTCAACAGG(+ACTCAC)GTACCTCCTCTGAACAACAAGCTTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240604225644_tvzx TGAGAATGTGGGAAGCTTGTTGTTCAGAGGAG(+ACTCAC)GTACCCTGTTGAGAAAATCTTCAAATGAGAG NGG 10 8 17 8 24 0 100 5 10
    240604225643_oraa TGAAATGGCCTCAGCAAGAATAAAAGATGAAG(+ACTCAC)GATCCTGTGGACTTTCTTCTCCTGCTACAAA NGG 10 8 17 8 24 0 100 5 10
    240604225641_h6km ATACATTTGTAGCAGGAGAAGAAAGTCCACAG(+ACTCAC)GATCCTTCATCTTTTATTCTTGCTGAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240604225639_26ju GCTGTCATCAGACACATCAGAAGAGGGCATCG(+ACTCAC)GATCCCATTACAGATGCTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604225638_izto ACCACATGGTGGCTCACAAGCATCTGTAATGG(+ACTCAC)GATCCGATGCCCTCTTCTGATGTGTCTGATG NGG 10 8 17 8 24 0 100 5 10
    240604225636_2y13 CCATTATGACCCCGTTTCCACACATCCGCCTG(+ACTCAC)GTACCGTGCTCACCATGAACTATGCAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240604225635_ih4s GCTTTCTTTCTGCATAGTTCATGGTGAGCACG(+ACTCAC)GTACCAGGCGGATGTGTGGAAACGGGGTCAT NGG 10 8 17 8 24 0 100 5 10