Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224857_jjs2 TGTGAGAATGGGGATTGATGAGGCCCAGCTTG(+ACTCAC)GTACCTGGAAAATGTGTATGGTAAGGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240604224855_2g4q TGCACATTTTCCTTACCATACACATTTTCCAG(+ACTCAC)GTACCAAGCTGGGCCTCATCAATCCCCATTC NGG 10 8 17 8 24 0 100 5 10
    240604224854_ssf6 AGAGACTGCCTGGAGATGGACAGAGAAGCTGG(+ACTCAC)GATCCTTGGTGACCAGCCCCTCTGAAACACT NGG 10 8 17 8 24 0 100 5 10
    240604224852_15y0 CCTAGAGTGTTTCAGAGGGGCTGGTCACCAAG(+ACTCAC)GATCCCAGCTTCTCTGTCCATCTCCAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240604224849_wjbo AACACTCCCTCAGTCCCAGAAGTTTCCCCACG(+ACTCAC)GATCCCCATTTGCATATAACCTCTTCCCATC NGG 10 8 17 8 24 0 100 5 10
    240604224847_c205 GGTAGGATGGGAAGAGGTTATATGCAAATGGG(+ACTCAC)GATCCGTGGGGAAACTTCTGGGACTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240604224845_v23d AGGAAATGGGAGGGAGATGAGAAAACAGGATG(+ACTCAC)GTACCAATCACCTGGGTAATCAACTGGTTGA NGG 10 8 17 8 24 0 100 5 10
    240604224844_g5ld CCTCTTCAACCAGTTGATTACCCAGGTGATTG(+ACTCAC)GTACCATCCTGTTTTCTCATCTCCCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240604224842_yspv ACTACATGGTGGCTTGCAATCATCTGTAATGG(+ACTCAC)GATCCAATGCCTTCTTTGGGTGAATCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604224841_a5zv GCTATCTTCAGATTCACCCAAAGAAGGCATTG(+ACTCAC)GATCCCATTACAGATGATTGCAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10