Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224913_88yk GCTGTTTTCAGACACACCAGAAGAGGGCATCG(+ACTCAC)GATCCCATCACACATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604224912_f60s ACCACATGGTGGCTCACAACCATGTGTGATGG(+ACTCAC)GATCCGATGCCCTCTTCTGGTGTGTCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240604224910_scre GGCTCACAATGCCTGTACCTCCAGCTTTGAGG(+ACTCAC)GATCCTTTATTCAGGTGTACTTGGACACATG NGG 10 8 17 8 24 0 100 5 10
    240604224909_xzu3 GTCTACATGTGTCCAAGTACACCTGAATAAAG(+ACTCAC)GATCCCTCAAAGCTGGAGGTACAGGCATTGT NGG 10 8 17 8 24 0 100 5 10
    240604224907_2ue0 TGATTTCTGTGGGGGCTCAGAAGAGGGCACTG(+ACTCAC)GATCCCTGGAGCTGGAGTTACAGATGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240604224905_ujlf AGCTCACGGCCATCTGTAACTCCAGCTCCAGG(+ACTCAC)GATCCAGTGCCCTCTTCTGAGCCCCCACAGA NGG 10 8 17 8 24 0 100 5 10
    240604224903_3ioc GTTCATGGTCCTGTGTACAGTGTCAGGCATGG(+ACTCAC)GTACCAGTTCTTTGAGTGCCAATTACATATT NGG 10 8 17 8 24 0 100 5 10
    240604224902_ubq7 CAACCAATATGTAATTGGCACTCAAAGAACTG(+ACTCAC)GTACCCATGCCTGACACTGTACACAGGACCA NGG 10 8 17 8 24 0 100 5 10
    240604224900_5p6r AGAATCCCTTGTTTGGCTAAGAGCCTGGAGAG(+ACTCAC)GATCCCCTCTTCCCAAATAATTCCTCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240604224858_z2t2 ATAGAAGGCAGAGGAATTATTTGGGAAGAGGG(+ACTCAC)GATCCTCTCCAGGCTCTTAGCCAAACAAGGG NGG 10 8 17 8 24 0 100 5 10