Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225026_kd5o GGATGGAGCATCTAGAGACTGTCATACCCGGG(+ACTCAC)GATCCATCCCATTATTAGCTCCAAACGCTGA NGG 10 8 17 8 24 0 100 5 10
    240604225025_nsrd TGGTTTCAGCGTTTGGAGCTAATAATGGGATG(+ACTCAC)GATCCCCGGGTATGACAGTCTCTAGATGCTC NGG 10 8 17 8 24 0 100 5 10
    240604225024_td5k ACTCATAACCTAGCTAAGAATTTTTGAGTCTG(+ACTCAC)GTACCCATGGAGCACAAAAGCATTACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604225021_0cfq ATACATTTCTGTAATGCTTTTGTGCTCCATGG(+ACTCAC)GTACCAGACTCAAAAATTCTTAGCTAGGTTA NGG 10 8 17 8 24 0 100 5 10
    240604225019_d420 AGACTCAGGAAAGAGGTACTTGGGGTCTGTGG(+ACTCAC)GTACCCAGATATATCTAAAAATACATAGACA NGG 10 8 17 8 24 0 100 5 10
    240604225018_63wy CCAGCTGTCTATGTATTTTTAGATATATCTGG(+ACTCAC)GTACCCACAGACCCCAAGTACCTCTTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240604225017_a3cy TCAACTGACTACGTAGCTGACCTAGAGCTTTG(+ACTCAC)GATCCTCCACCTCCACTTCCCCAGTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240604225015_615r TACAGGCCAGCACTGGGGAAGTGGAGGTGGAG(+ACTCAC)GATCCAAAGCTCTAGGTCAGCTACGTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240604225014_6wp8 CACCATTTGAGGCACAAAATCCAAACTTACAG(+ACTCAC)GATCCGATATTATGTTGATCTGACAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240604225012_tgha AGCCCAGAAACTGTCAGATCAACATAATATCG(+ACTCAC)GATCCTGTAAGTTTGGATTTTGTGCCTCAAA NGG 10 8 17 8 24 0 100 5 10