Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225117_br6q GACCATTGTAGCTCATAGTTAAATATGTTAAG(+ACTCAC)GATCCAGCATCTCAAAGATTAAAAAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240604225116_nbu0 TTTTTTTTATTTTTTTAATCTTTGAGATGCTG(+ACTCAC)GATCCTTAACATATTTAACTATGAGCTACAA NGG 10 8 17 8 24 0 100 5 10
    240604225114_oz5y TGCAGAGAAGAGGCTAATAATGAACTCAGAGG(+ACTCAC)GTACCTTGAAGACTGGTGTGCCAATGGATAC NGG 10 8 17 8 24 0 100 5 10
    240604225112_z6ns AACTAGTATCCATTGGCACACCAGTCTTCAAG(+ACTCAC)GTACCCTCTGAGTTCATTATTAGCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604225111_54je GTGACGCTCAGATCTGAGAAGAAACATGATGG(+ACTCAC)GATCCATATCAGGTTGTGAATATTTTTTTGA NGG 10 8 17 8 24 0 100 5 10
    240604225109_d4dv TGTTCTCAAAAAAATATTCACAACCTGATATG(+ACTCAC)GATCCCATCATGTTTCTTCTCAGATCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240604225108_bcrf TAAAGTACTATGACACTAGAACCTTTGCAGAG(+ACTCAC)GATCCAACTACAATGTAAGGTTGATCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240604225107_thm3 GATACTGGAAGATCAACCTTACATTGTAGTTG(+ACTCAC)GATCCTCTGCAAAGGTTCTAGTGTCATAGTA NGG 10 8 17 8 24 0 100 5 10
    240604225104_oabu AGAGCGGTCACTGGACTAGGATGCATCCCTTG(+ACTCAC)GATCCTGGGATAGGCTCTGATCGGACCATAA NGG 10 8 17 8 24 0 100 5 10
    240604225102_l4pg AACACTTATGGTCCGATCAGAGCCTATCCCAG(+ACTCAC)GATCCAAGGGATGCATCCTAGTCCAGTGACC NGG 10 8 17 8 24 0 100 5 10