Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225044_n5sa GTAAGGAAGCAGAGCCCAGGAGCTCAAAGCTG(+ACTCAC)GTACCCAGAAGAGCCAGCAGGGCCAGGCTAA NGG 10 8 17 8 24 0 100 5 10
    240604225042_imt6 AGTCCTTAGCCTGGCCCTGCTGGCTCTTCTGG(+ACTCAC)GTACCAGCTTTGAGCTCCTGGGCTCTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240604225040_fd4d GGTGACCTGCCATCTGTGACAAGCTTTCTCGG(+ACTCAC)GTACCTCTCCCCACCTCATTCCTTTAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240604225039_cn1n CTCCATAGCCTAAAGGAATGAGGTGGGGAGAG(+ACTCAC)GTACCCGAGAAAGCTTGTCACAGATGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240604225038_6v9j TGTAATTTTTGTATATAATGGGCCAAATTAAG(+ACTCAC)GATCCTGCTCTCTTTCTTTTGGAGGTAGTTT NGG 10 8 17 8 24 0 100 5 10
    240604225036_nami ACCTCAAACTACCTCCAAAAGAAAGAGAGCAG(+ACTCAC)GATCCTTAATTTGGCCCATTATATACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604225034_g2qk GCATGTATTCAGTTTTAGAATCACTTTTACTG(+ACTCAC)GATCCTGTAGTAATGTCTAGAAGTATCATAT NGG 10 8 17 8 24 0 100 5 10
    240604225033_hggp AATAGATATGATACTTCTAGACATTACTACAG(+ACTCAC)GATCCAGTAAAAGTGATTCTAAAACTGAATA NGG 10 8 17 8 24 0 100 5 10
    240604225031_f149 TTACTTGAAAATTGGACATAGTACTACCAGAG(+ACTCAC)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240604225030_4syi TTCTGGATATATGCCCAGGAGAGGTATTGTGG(+ACTCAC)GATCCTCTGGTAGTACTATGTCCAATTTTCA NGG 10 8 17 8 24 0 100 5 10