Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225059_9avq TTTTAAGACTAGCCACAGGCTATGAATATATG(+ACTCAC)GTACCCCTTTGAAGGTAAAAATTGAAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240604225057_6p33 AAAACATTGCTTCAATTTTTACCTTCAAAGGG(+ACTCAC)GTACCATATATTCATAGCCTGTGGCTAGTCT NGG 10 8 17 8 24 0 100 5 10
    240604225056_nffc GATTAAATTCGGGTCATCAGGATTGGCAAGAG(+ACTCAC)GTACCTTTACCTGCTGAGTCATTGCATTGGC NGG 10 8 17 8 24 0 100 5 10
    240604225055_0gm8 ATTAGGCCAATGCAATGACTCAGCAGGTAAAG(+ACTCAC)GTACCTCTTGCCAATCCTGATGACCCGAATT NGG 10 8 17 8 24 0 100 5 10
    240604225053_lu88 GATGGTCTTCGACTGTAAAATGGGGTCTGTGG(+ACTCAC)GTACCATCATTCTCTGGGCCGGTTGTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240604225051_7e2r ACACCTCAGGACAACCGGCCCAGAGAATGATG(+ACTCAC)GTACCCACAGACCCCATTTTACAGTCGAAGA NGG 10 8 17 8 24 0 100 5 10
    240604225050_aa9y AAAAGGTATCTGGGTAGCTCAAGCCAAGCAGG(+ACTCAC)GATCCTGGTTATTTCTGTTGGCTCTTTAACA NGG 10 8 17 8 24 0 100 5 10
    240604225047_7lkz AATGATGTTAAAGAGCCAACAGAAATAACCAG(+ACTCAC)GATCCCTGCTTGGCTTGAGCTACCCAGATAC NGG 10 8 17 8 24 0 100 5 10
    240604225046_n69f TTCAATCTCAGTCTTTCTAGGGGAAAAAACTG(+ACTCAC)GATCCAGAATTACTCAGACACCTTTCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240604225045_mgrf CTGAGAAAGAGAAAGGTGTCTGAGTAATTCTG(+ACTCAC)GATCCAGTTTTTTCCCCTAGAAAGACTGAGA NGG 10 8 17 8 24 0 100 5 10