Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225226_ayd1 AGGCTCTGAATCACAAGTGGACCCGCCCTCAG(+ACTCAC)GATCCAGAGGGAACTGCTCGGATGCCTTACG NGG 10 8 17 8 24 0 100 5 10
    240604225224_vkjh GTGAGCGTAAGGCATCCGAGCAGTTCCCTCTG(+ACTCAC)GATCCTGAGGGCGGGTCCACTTGTGATTCAG NGG 10 8 17 8 24 0 100 5 10
    240604225223_czhu CTAAGCACTGGGCCATGAAATCTGACACTAAG(+ACTCAC)GTACCCTGAGCATAGGAGGGGCATGGGTCGC NGG 10 8 17 8 24 0 100 5 10
    240604225221_i8bv ATGGTGCGACCCATGCCCCTCCTATGCTCAGG(+ACTCAC)GTACCTTAGTGTCAGATTTCATGGCCCAGTG NGG 10 8 17 8 24 0 100 5 10
    240604225219_mxoc GACCTGCATCCCCTAAGTGAGGACCCTACAGG(+ACTCAC)GTACCTTGTGCTCTGCCCCCAGACCCATAGC NGG 10 8 17 8 24 0 100 5 10
    240604225218_x14b CTAAGGCTATGGGTCTGGGGGCAGAGCACAAG(+ACTCAC)GTACCCTGTAGGGTCCTCACTTAGGGGATGC NGG 10 8 17 8 24 0 100 5 10
    240604225216_c2gi TCCTTGGGGGTCTGGCAGGTGGCCACACCACG(+ACTCAC)GATCCTTGAGCTGTCTCGAGGGTCAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604225215_xfft TGCCACTGGCCTGACCCTCGAGACAGCTCAAG(+ACTCAC)GATCCGTGGTGTGGCCACCTGCCAGACCCCC NGG 10 8 17 8 24 0 100 5 10
    240604225212_g4hx GCTGGCTGCTCTCCTCCCTGCAGTCCACCCTG(+ACTCAC)GATCCCTCGCCTCTAGGGAACAGAAGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240604225210_1d0u CCAGCGTAAACTTCTGTTCCCTAGAGGCGAGG(+ACTCAC)GATCCAGGGTGGACTGCAGGGAGGAGAGCAG NGG 10 8 17 8 24 0 100 5 10