Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225301_0zfo TAATAGTTGCAATGTAATTTATGGAGCACTTG(+ACTCAC)GTACCACATTGTGTCTATTCTTTCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240604225300_8f61 CACAAGCAGCTTGAAAGAATAGACACAATGTG(+ACTCAC)GTACCAAGTGCTCCATAAATTACATTGCAAC NGG 10 8 17 8 24 0 100 5 10
    240604225258_5umc ACATTGTCTGTGTACATTCTTTCAAACTCCTG(+ACTCAC)GTACCCTCCACACACTGCTCTGCTTACTATT NGG 10 8 17 8 24 0 100 5 10
    240604225255_brm6 GCAACAATAGTAAGCAGAGCAGTGTGTGGAGG(+ACTCAC)GTACCAGGAGTTTGAAAGAATGTACACAGAC NGG 10 8 17 8 24 0 100 5 10
    240604225253_5o7z ATCAGCATGCCTGCCTCATGGGATTAAAGGTG(+ACTCAC)GTACCACCATGCCTGGATCTAATTAAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604225251_g0mn TCTACCCAGCTTAATTAGATCCAGGCATGGTG(+ACTCAC)GTACCACCTTTAATCCCATGAGGCAGGCATG NGG 10 8 17 8 24 0 100 5 10
    240604225249_q2qn GTCAGGCCATTCACAGCAATATACCACTCCTG(+ACTCAC)GTACCAACTTCTGTCTTAGTTAGGGTTTTAC NGG 10 8 17 8 24 0 100 5 10
    240604225248_gil9 CAGCAGTAAAACCCTAACTAAGACAGAAGTTG(+ACTCAC)GTACCAGGAGTGGTATATTGCTGTGAATGGC NGG 10 8 17 8 24 0 100 5 10
    240604225246_xnfz CATTAATTACTAAAGCTCTTTATATAGGAGAG(+ACTCAC)GATCCTCTTGAGATTTGTCCATCAATGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604225245_c5pj ACATGCCAGCATTGATGGACAAATCTCAAGAG(+ACTCAC)GATCCTCTCCTATATAAAGAGCTTTAGTAAT NGG 10 8 17 8 24 0 100 5 10