Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225243_fdwd ATTATGTAAACTCTAGACCCCGAAACTTCCAG(+ACTCAC)GATCCTCTGTTCTCAGATGCTCTGGTGATCT NGG 10 8 17 8 24 0 100 5 10
    240604225242_du2d TAGGCAGATCACCAGAGCATCTGAGAACAGAG(+ACTCAC)GATCCTGGAAGTTTCGGGGTCTAGAGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240604225240_9a1r TCACTCAGGATATTGTTACAGGACATGCAGGG(+ACTCAC)GATCCAATCCCTTCCACACCTTCCGACCCTT NGG 10 8 17 8 24 0 100 5 10
    240604225239_iapi AGGAGAAGGGTCGGAAGGTGTGGAAGGGATTG(+ACTCAC)GATCCCCTGCATGTCCTGTAACAATATCCTG NGG 10 8 17 8 24 0 100 5 10
    240604225237_wxlr AACAACTAAGATGCTTCATTGTTTCATTTGAG(+ACTCAC)GATCCCAGATCACACAGCACACAAGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240604225236_bfiu AGGTACCTGTCCTTGTGTGCTGTGTGATCTGG(+ACTCAC)GATCCTCAAATGAAACAATGAAGCATCTTAG NGG 10 8 17 8 24 0 100 5 10
    240604225233_8ki2 ACTCTTAAGCTTGACAGGCGGTTGAAGCCCTG(+ACTCAC)GTACCCACATCTGGGGCTAGGTGAACAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604225231_eq1r GTACCCTGCTGTTCACCTAGCCCCAGATGTGG(+ACTCAC)GTACCAGGGCTTCAACCGCCTGTCAAGCTTA NGG 10 8 17 8 24 0 100 5 10
    240604225230_ucaw TTGAGATACCGAATGCCTCCCTTTGTGTTTAG(+ACTCAC)GATCCTCAGGACGCTTGGCTCCAGTGCTTGC NGG 10 8 17 8 24 0 100 5 10
    240604225228_ndyk AGGAAGCAAGCACTGGAGCCAAGCGTCCTGAG(+ACTCAC)GATCCTAAACACAAAGGGAGGCATTCGGTAT NGG 10 8 17 8 24 0 100 5 10