Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604225319_2wsu CTTTTCAGATAGCCCTGGCTGGTGGGGGAATG(+ACTCAC)GATCCCTGAGAACACGGGCATGTCACACAGG NGG 10 8 17 8 24 0 100 5 10
    240604225317_2okb CATTCCCTGTGTGACATGCCCGTGTTCTCAGG(+ACTCAC)GATCCATTCCCCCACCAGCCAGGGCTATCTG NGG 10 8 17 8 24 0 100 5 10
    240604225315_hqyy GCAGGGGGAGAGAGGAGCTGAGGAAATGTGGG(+ACTCAC)GATCCTGGGGGCTCGGACCGGGGTGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240604225311_hpbo ACATAGACACTCACCCCGGTCCGAGCCCCCAG(+ACTCAC)GATCCCCACATTTCCTCAGCTCCTCTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240604225310_wi7o CTATAGATGGAGATCTGAAGATTAGACTCTAG(+ACTCAC)GATCCACAGATGAGAGAGTATACGTGACATT NGG 10 8 17 8 24 0 100 5 10
    240604225309_j6he AGGCAAATGTCACGTATACTCTCTCATCTGTG(+ACTCAC)GATCCTAGAGTCTAATCTTCAGATCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240604225307_lbla CCTCACCAGGTAGCTCTCTAGCCCCAAGAAAG(+ACTCAC)GATCCCACCAGAAACAGTATCAGATGTCATA NGG 10 8 17 8 24 0 100 5 10
    240604225305_nx6n TAATTTATGACATCTGATACTGTTTCTGGTGG(+ACTCAC)GATCCTTTCTTGGGGCTAGAGAGCTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240604225304_b9uu CAAGCTGCTGAAACCTTTAAAATGTATCAAGG(+ACTCAC)GATCCATAGCCTCTACCATCCGGAATGCTAA NGG 10 8 17 8 24 0 100 5 10
    240604225302_gu1f ATCCTTTAGCATTCCGGATGGTAGAGGCTATG(+ACTCAC)GATCCCTTGATACATTTTAAAGGTTTCAGCA NGG 10 8 17 8 24 0 100 5 10