Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224825_x1jn AATTGTAACATAGGTAGTATGCCTTCACACTG(+ACTCAC)GATCCCGGGGGCATGGGTTTTGGAGATGTTA NGG 10 8 17 8 24 0 100 5 10
    240604224822_flul CAAAATAACATCTCCAAAACCCATGCCCCCGG(+ACTCAC)GATCCAGTGTGAAGGCATACTACCTATGTTA NGG 10 8 17 8 24 0 100 5 10
    240604224821_3iu8 CACCACCTTGCTACGTGCATGTACCAGGCAAG(+ACTCAC)GTACCAAAGATAACAAATGAAAAACAAACCC NGG 10 8 17 8 24 0 100 5 10
    240604224820_6p7r ATCATGGGTTTGTTTTTCATTTGTTATCTTTG(+ACTCAC)GTACCTTGCCTGGTACATGCACGTAGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240604224818_dtej AATCAGCTTGCTTTGTTGGCCTAGATATTTTG(+ACTCAC)GATCCTTTATTGAAATGAGGCAGTGAGATAG NGG 10 8 17 8 24 0 100 5 10
    240604224817_qn5e GGAAACTATCTCACTGCCTCATTTCAATAAAG(+ACTCAC)GATCCAAAATATCTAGGCCAACAAAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240604224816_ywk7 AGTGGAAATAATTTGCTGTCGTTTCAGACTTG(+ACTCAC)GATCCTACAAGCCTGTCCGTTCTGTAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604224814_ffgz CAGTACCACTTACAGAACGGACAGGCTTGTAG(+ACTCAC)GATCCAAGTCTGAAACGACAGCAAATTATTT NGG 10 8 17 8 24 0 100 5 10
    240604224813_2xtc TGACAAGGGAGTCGGAAGCTAAGGAGGTGACG(+ACTCAC)GATCCTAATGCTTGTCAAATAAACCGATCGC NGG 10 8 17 8 24 0 100 5 10
    240604224812_5ekt TTTTGGCGATCGGTTTATTTGACAAGCATTAG(+ACTCAC)GATCCGTCACCTCCTTAGCTTCCGACTCCCT NGG 10 8 17 8 24 0 100 5 10