Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224740_l7ph GGTAGAGTGCCCCCAAGTGGCAGGTAGCAGTG(+ACTCAC)GTACCAATCATATGACCCAGATAAGCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240604224738_9170 TAGAGGGAGGGCTTATCTGGGTCATATGATTG(+ACTCAC)GTACCACTGCTACCTGCCACTTGGGGGCACT NGG 10 8 17 8 24 0 100 5 10
    240604224736_iz1t GTGACAGTCTCTCCCTTCCCTGCTTGCCGTAG(+ACTCAC)GATCCGTGCAAATGTAGTTTGAAGATGCAAC NGG 10 8 17 8 24 0 100 5 10
    240604224735_q3yy TGGTGGTTGCATCTTCAAACTACATTTGCACG(+ACTCAC)GATCCTACGGCAAGCAGGGAAGGGAGAGACT NGG 10 8 17 8 24 0 100 5 10
    240604224733_4eh2 CATTTGCTTTCCTTTGCCCTTAAATAAATATG(+ACTCAC)GTACCAGAAAGCCCACATTGTGAGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240604224732_2gjj TGCATAAGACAACTCACAATGTGGGCTTTCTG(+ACTCAC)GTACCATATTTATTTAAGGGCAAAGGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240604224731_ifdt ACCAAATATACAGCATCAATAAAATGTACACG(+ACTCAC)GTACCATGAAAAATTCGAGAACTCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240604224730_feu7 GAAAAGCAGCTTGAGTTCTCGAATTTTTCATG(+ACTCAC)GTACCGTGTACATTTTATTGATGCTGTATAT NGG 10 8 17 8 24 0 100 5 10
    240604224728_pju3 ACTGCTGGTGTATCTGTGAAGTGTTTGCAGTG(+ACTCAC)GATCCATCTGCCGCTGCCTGCTAACATATGA NGG 10 8 17 8 24 0 100 5 10
    240604224727_k3fp TTAGTTCATATGTTAGCAGGCAGCGGCAGATG(+ACTCAC)GATCCACTGCAAACACTTCACAGATACACCA NGG 10 8 17 8 24 0 100 5 10