Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224707_rnro AACATAATTAGAAAGTCTCCTTCTGGCTGATG(+ACTCAC)GTACCACTGGTCTCTCACCACACTAAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240604224704_qsk5 TGGCAAAACCTTAGTGTGGTGAGAGACCAGTG(+ACTCAC)GTACCATCAGCCAGAAGGAGACTTTCTAATT NGG 10 8 17 8 24 0 100 5 10
    240604224703_1z4b GCTAGTCCTATACTTTTACTTAATCTTCTCTG(+ACTCAC)GTACCAGGTAATATTCTCTTTCTTTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240604224701_grn1 CTGGGCAGGAGAAAGAAAGAGAATATTACCTG(+ACTCAC)GTACCAGAGAAGATTAAGTAAAAGTATAGGA NGG 10 8 17 8 24 0 100 5 10
    240604224700_4oiv TTAGAAACAGTCTCAGACTTTTTTACAGAAAG(+ACTCAC)GTACCGATAAACAATACACCCTGAGTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240604224659_e4gj AGTCTTGAAGACTCAGGGTGTATTGTTTATCG(+ACTCAC)GTACCTTTCTGTAAAAAAGTCTGAGACTGTT NGG 10 8 17 8 24 0 100 5 10
    240604224657_knmk CGCTTTTTCTTCTTCTTTTAATTTCTCTATGG(+ACTCAC)GATCCTGGAGTTCTGGTTCCTTACTGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240604224656_0ltn GGGTTGCCTTCAGTAAGGAACCAGAACTCCAG(+ACTCAC)GATCCCATAGAGAAATTAAAAGAAGAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604224654_tgni AAATAACCTAGAATCATCTCCAGACTCTAAAG(+ACTCAC)GATCCTATTCATTGTTTCCCCCTCCCCAAGG NGG 10 8 17 8 24 0 100 5 10
    240604224653_7b4y GTTGACCTTGGGGAGGGGGAAACAATGAATAG(+ACTCAC)GATCCTTTAGAGTCTGGAGATGATTCTAGGT NGG 10 8 17 8 24 0 100 5 10