Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224619_zou9 CAAGGTTTGAGGGTATTTGAATAAAATTTTTG(+ACTCAC)GATCCAGCTGCAACATAAAGAGTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604224617_y717 ATGTGGGAAGGGCACTCTTTATGTTGCAGCTG(+ACTCAC)GATCCAAAAATTTTATTCAAATACCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240604224616_0qvf AGTGCCAGGCCTTGGGTCCTCCCCTTGAGATG(+ACTCAC)GATCCCACTTCAGGCCTGTCAGTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240604224614_nbjp GAAAAGAAGGTCCACTGACAGGCCTGAAGTGG(+ACTCAC)GATCCATCTCAAGGGGAGGACCCAAGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604224613_8nh8 CAAGGTTTGAGGATATTTGAATAAAATTTTTG(+ACTCAC)GATCCAGCTGCAACATAAAGAGTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604224612_sw3q ATGTGGGAAGGGCACTCTTTATGTTGCAGCTG(+ACTCAC)GATCCAAAAATTTTATTCAAATATCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240604224610_4gz6 TGAAGCAATAGAAACATGCGATTTAGCTTTGG(+ACTCAC)GATCCTCTGAATGAGGGATTCCCCCAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240604224609_8hwa TTATAGCCTTTGGGGGAATCCCTCATTCAGAG(+ACTCAC)GATCCCAAAGCTAAATCGCATGTTTCTATTG NGG 10 8 17 8 24 0 100 5 10
    240604224606_ook3 AGCCCCCTCCCCCATCGAATCATCCCCCCAGG(+ACTCAC)GTACCCCAGCTAGTCACATACAAAACAGTTT NGG 10 8 17 8 24 0 100 5 10
    240604224604_d36v AGACAAAACTGTTTTGTATGTGACTAGCTGGG(+ACTCAC)GTACCCTGGGGGGATGATTCGATGGGGGAGG NGG 10 8 17 8 24 0 100 5 10