Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224512_dkg0 ATAGAAAAGGGTGGTATCTGGGAGTCTAAGGG(+ACTCAC)GTACCTTTTCATCTGACCTACTGACCAAAGT NGG 10 8 17 8 24 0 100 5 10
    240604224510_4a93 CTATGACTTTGGTCAGTAGGTCAGATGAAAAG(+ACTCAC)GTACCCCTTAGACTCCCAGATACCACCCTTT NGG 10 8 17 8 24 0 100 5 10
    240604224508_finq TCCATGCTACCTACCAATAAAACCAGGCCCTG(+ACTCAC)GTACCAAAGGCCTGTGGAGTACTGGGCACTG NGG 10 8 17 8 24 0 100 5 10
    240604224506_shg6 CAGCGCAGTGCCCAGTACTCCACAGGCCTTTG(+ACTCAC)GTACCAGGGCCTGGTTTTATTGGTAGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240604224504_76b0 GAGTGCTGATAAAGGCATCTGGGGGCCCAGAG(+ACTCAC)GATCCCAGTAGAGACTGCTTGCTCTATGTAT NGG 10 8 17 8 24 0 100 5 10
    240604224502_6to4 CTGTAATACATAGAGCAAGCAGTCTCTACTGG(+ACTCAC)GATCCTCTGGGCCCCCAGATGCCTTTATCAG NGG 10 8 17 8 24 0 100 5 10
    240604224501_oure GTTACTGTTCATTTCTGGAGTCTGATGTTCTG(+ACTCAC)GTACCCAGCTCTGGTGTCCCTGCCAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240604224459_xoh6 TTTCTCAGCCCTGGCAGGGACACCAGAGCTGG(+ACTCAC)GTACCAGAACATCAGACTCCAGAAATGAACA NGG 10 8 17 8 24 0 100 5 10
    240604224457_018o GACACACTCCCTTGAGTTCTTGGGCAACTTGG(+ACTCAC)GTACCTGATGAGTGGCCTTTGAGCTTAACTG NGG 10 8 17 8 24 0 100 5 10
    240604224456_c8n2 TGTTTCAGTTAAGCTCAAAGGCCACTCATCAG(+ACTCAC)GTACCCAAGTTGCCCAAGAACTCAAGGGAGT NGG 10 8 17 8 24 0 100 5 10