Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224437_hsmm AAGTGGATTTCCGAGATGAGGGTTGGCACTTG(+ACTCAC)GTACCCACCTGTCGAGTTCTGTGGGACGTCA NGG 10 8 17 8 24 0 100 5 10
    240604224435_5oi9 GAGCATGACGTCCCACAGAACTCGACAGGTGG(+ACTCAC)GTACCAAGTGCCAACCCTCATCTCGGAAATC NGG 10 8 17 8 24 0 100 5 10
    240604224433_xzkh GCTCTGCATTACAAATGTCCTAATGGTGCCAG(+ACTCAC)GTACCTTCTGAAGACTCACAGGAGACATTAC NGG 10 8 17 8 24 0 100 5 10
    240604224432_3ljx CTTGAGTAATGTCTCCTGTGAGTCTTCAGAAG(+ACTCAC)GTACCTGGCACCATTAGGACATTTGTAATGC NGG 10 8 17 8 24 0 100 5 10
    240604224430_7jyo AAACAAACAATCTATGATAACTCTGTACAGAG(+ACTCAC)GTACCAAACCTTTAAGTATTTGTGATGGGAA NGG 10 8 17 8 24 0 100 5 10
    240604224428_hbvl TCAAATTCCCATCACAAATACTTAAAGGTTTG(+ACTCAC)GTACCTCTGTACAGAGTTATCATAGATTGTT NGG 10 8 17 8 24 0 100 5 10
    240604224427_ghf9 TAATTCAGGGGCTACAGCTCCAAGCCAAGCAG(+ACTCAC)GTACCAGGGCACGATGGATCGAAAGGCAATA NGG 10 8 17 8 24 0 100 5 10
    240604224424_e3bu CCACATATTGCCTTTCGATCCATCGTGCCCTG(+ACTCAC)GTACCTGCTTGGCTTGGAGCTGTAGCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604224422_6k9h GGCATGGCCTTTCCTCAGAACTTTCTACCATG(+ACTCAC)GATCCAAAGCCTAAGACTAGGCCATCGTAGA NGG 10 8 17 8 24 0 100 5 10
    240604224420_lrwq GATGGTCTACGATGGCCTAGTCTTAGGCTTTG(+ACTCAC)GATCCATGGTAGAAAGTTCTGAGGAAAGGCC NGG 10 8 17 8 24 0 100 5 10