Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224346_zmo0 GCACCGAGTGGGGAGGAGGGAGAGGGATGACG(+ACTCAC)GTACCGCACTGTCCACATCTCCCTGGGCCCC NGG 10 8 17 8 24 0 100 5 10
    240604224345_wz1g GACGGGGGGCCCAGGGAGATGTGGACAGTGCG(+ACTCAC)GTACCGTCATCCCTCTCCCTCCTCCCCACTC NGG 10 8 17 8 24 0 100 5 10
    240604224343_b2l8 CCACACTGGATAAGATGTGTGAGCTCCCAGCG(+ACTCAC)GATCCTTCTCTCAAGCACCAAGCCCCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240604224341_ckrm TTTTAAAACTGGGGCTTGGTGCTTGAGAGAAG(+ACTCAC)GATCCGCTGGGAGCTCACACATCTTATCCAG NGG 10 8 17 8 24 0 100 5 10
    240604224339_hd71 TCTCTTATTACCTAATAAAATGTAGCATCCTG(+ACTCAC)GTACCTCTGTGATGTCTGGCGCCCATGCCCT NGG 10 8 17 8 24 0 100 5 10
    240604224338_emmh CACAAAGGGCATGGGCGCCAGACATCACAGAG(+ACTCAC)GTACCAGGATGCTACATTTTATTAGGTAATA NGG 10 8 17 8 24 0 100 5 10
    240604224336_izrj TGACCAAGTTTGCAGCATTGCTGGTGATGTGG(+ACTCAC)GATCCGTGGGGTTTTTGTTTCCCAAGACAGG NGG 10 8 17 8 24 0 100 5 10
    240604224333_y0d3 GAAACCCTGTCTTGGGAAACAAAAACCCCACG(+ACTCAC)GATCCCACATCACCAGCAATGCTGCAAACTT NGG 10 8 17 8 24 0 100 5 10
    240604224332_c2hv GTGACTTAAATGGGGTCTCCCGGTTTCAAGTG(+ACTCAC)GATCCTCACTCCTGCCCGTTCTTAGCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240604224330_w9st GGAGGTCCCTGCTAAGAACGGGCAGGAGTGAG(+ACTCAC)GATCCACTTGAAACCGGGAGACCCCATTTAA NGG 10 8 17 8 24 0 100 5 10