Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224329_te16 GATGCTGCACTATTGAACAAGAAAACAGTAAG(+ACTCAC)GTACCACCAACACAACCCTCTTGTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604224327_x4fz TTTTTTCCTTTGACAAGAGGGTTGTGTTGGTG(+ACTCAC)GTACCTTACTGTTTTCTTGTTCAATAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240604224326_46p9 GATGGACCTCACCCCTTTTCCACAGGTCACTG(+ACTCAC)GATCCCACTGTCACCCTCGGTGCTTACACAT NGG 10 8 17 8 24 0 100 5 10
    240604224324_ct8m CTGAAATGTGTAAGCACCGAGGGTGACAGTGG(+ACTCAC)GATCCAGTGACCTGTGGAAAAGGGGTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240604224322_jhgp AAATCACTGCCCCATACTCAAACGACTCCCAG(+ACTCAC)GTACCCATTACTAGAAGTTTCTAAGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240604224320_atrd GGAGCCTGTGACTTAGAAACTTCTAGTAATGG(+ACTCAC)GTACCTGGGAGTCGTTTGAGTATGGGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240604224319_yr1m AAGCCACTTAAAGACCTATACACAACGTGATG(+ACTCAC)GTACCTTAGAATTCTGTAGTTGGGGAATTTC NGG 10 8 17 8 24 0 100 5 10
    240604224318_8vl7 AATGTGAAATTCCCCAACTACAGAATTCTAAG(+ACTCAC)GTACCATCACGTTGTGTATAGGTCTTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240604224316_iyim CTGTCTCTGCATCAGCTGACCATCTTCCAGTG(+ACTCAC)GTACCTCTCCTCCCCTGCTCACCCCTCACCA NGG 10 8 17 8 24 0 100 5 10
    240604224314_vsg2 CCATCTGGTGAGGGGTGAGCAGGGGAGGAGAG(+ACTCAC)GTACCACTGGAAGATGGTCAGCTGATGCAGA NGG 10 8 17 8 24 0 100 5 10