Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224223_16ll TCCCCAAAATCGTTTGAATTAGATTCTGCCTG(+ACTCAC)GTACCTGTGTTCTGATTGGTCCTGGAGTATA NGG 10 8 17 8 24 0 100 5 10
    240604224222_c3c9 CCTCGTATACTCCAGGACCAATCAGAACACAG(+ACTCAC)GTACCAGGCAGAATCTAATTCAAACGATTTT NGG 10 8 17 8 24 0 100 5 10
    240604224221_n9xa TGAAAGCATTACTATATTTTTTACATTAAGTG(+ACTCAC)GTACCAATAAAGTATTTTGTTACCAAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240604224219_wz16 CATTTAGCTTTTGGTAACAAAATACTTTATTG(+ACTCAC)GTACCACTTAATGTAAAAAATATAGTAATGC NGG 10 8 17 8 24 0 100 5 10
    240604224218_q0sl CTTCAAAGTCACGGGCCAGCCAGACACAAAAG(+ACTCAC)GTACCAGTCAAAGTCGCATTGTGGGAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240604224217_fs6v ACAGTCTAGCTCCCACAATGCGACTTTGACTG(+ACTCAC)GTACCTTTTGTGTCTGGCTGGCCCGTGACTT NGG 10 8 17 8 24 0 100 5 10
    240604224215_hq1w GTTTCGGAAGTTGATTCATGTAGCTGACAGTG(+ACTCAC)GATCCAGAAGGGTCAGTCCCCTTCACTCCAT NGG 10 8 17 8 24 0 100 5 10
    240604224214_8xab ACAAGATGGAGTGAAGGGGACTGACCCTTCTG(+ACTCAC)GATCCACTGTCAGCTACATGAATCAACTTCC NGG 10 8 17 8 24 0 100 5 10
    240604224212_h76q ATAGTACCCATGGTGTGTTCTGATCGATCCTG(+ACTCAC)GATCCCCGGACTCCTTGGGACATGCTATTGA NGG 10 8 17 8 24 0 100 5 10
    240604224210_ow21 CTGCTTCAATAGCATGTCCCAAGGAGTCCGGG(+ACTCAC)GATCCAGGATCGATCAGAACACACCATGGGT NGG 10 8 17 8 24 0 100 5 10