Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224136_4f2a CCTTTCTCAGTCACAAGCCATGACGACATGGG(+ACTCAC)GATCCGGACGCTTTGTGCTCTTAGCTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240604224134_r7ax CGGCCACAGAAGCTAAGAGCACAAAGCGTCCG(+ACTCAC)GATCCCCATGTCGTCATGGCTTGTGACTGAG NGG 10 8 17 8 24 0 100 5 10
    240604224132_g9wb GAAGCTCAGGCAGAAAGCCAACGGCTCCCAAG(+ACTCAC)GATCCACCCTGAGGAGCTAAGCAACGCGCTC NGG 10 8 17 8 24 0 100 5 10
    240604224130_ihwl TGAGCGAGCGCGTTGCTTAGCTCCTCAGGGTG(+ACTCAC)GATCCTTGGGAGCCGTTGGCTTTCTGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240604224128_jhgr ATGCAACACTGGGTGTTCACCTGCCTTACTGG(+ACTCAC)GATCCCATGTGTTTAGTAATTTAACGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240604224126_cxis TTCAGCTGGACGTTAAATTACTAAACACATGG(+ACTCAC)GATCCCAGTAAGGCAGGTGAACACCCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240604224125_neua AGACTGCTGGGAAACCCAGGCCAAAGGGCAGG(+ACTCAC)GATCCAGACTGGCCATCTCATTGTACACTGT NGG 10 8 17 8 24 0 100 5 10
    240604224123_x5n4 ATGACACAGTGTACAATGAGATGGCCAGTCTG(+ACTCAC)GATCCCTGCCCTTTGGCCTGGGTTTCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240604224122_n1zd GCATGGATTTGATCATGGGGGCCAGGGGCCAG(+ACTCAC)GTACCCCTGACCCTCTCAGTGTGGAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240604224119_tuj0 AGGTGCATCTCTCCACACTGAGAGGGTCAGGG(+ACTCAC)GTACCTGGCCCCTGGCCCCCATGATCAAATC NGG 10 8 17 8 24 0 100 5 10